Composite
Part:BBa_K568000:Design
Designed by: Team TU Munich 2011 Group: iGEM11_TU_Munich (2011-09-01)
Red light sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
- 1000COMPATIBLE WITH RFC[1000]
Source
This part was extracted from the original part BBa_K322127 by PCR.
Sequence
Sequence of first 1000 bp of PCR-Product shows one possible silent mutation in PcyA (Phycocyanobilin:ferredoxin oxidoreductase) gen on Position 45 T->C. Resulting triplet is CCC instead of CCT, which both code for proline.
References
[1] G A Gambetta and J C Lagarias, "Genetic engineering of phytochrome biosynthesis in bacteria." Proc Natl Acad Sci U S A 98(19):10566-71 (2001) PMID 11553807